PMID 39941151

Research
All papers

Differential Regulation of miRNA and Protein Profiles in Human Plasma-Derived Extracellular Vesicles via Continuous Aerobic and High-Intensity Interval Training

Wang Z, Ou Y, Zhu X, Zhou Y, Zheng X, Zhang M, Li S, Yang SN, Juntti-Berggren L, Berggren PO, Zheng X

Question

How do acute CAT and HIIT differentially regulate miRNA and protein profiles in plasma-derived extracellular vesicles?

Summary

In five healthy active men, single bouts of cycling HIIT and continuous aerobic training produced different extracellular-vesicle miRNA and protein profiles. The study is mechanistic and acute; it does not show training adaptation or clinical outcomes.

Methodology

  • Five healthy active male volunteers.
  • 5 participants.
  • Cycling.
  • Work intervals: 2 minutes.
  • Recovery: 2 minutes active recovery.
  • Intensity: 80%-95% HRmax work; about 70% HRmax recovery.
  • 20 minutes plus 2-minute dynamic stretch.
  • Single session.
  • Acute.
  • Acute within-participant exercise comparison with multi-omics analysis
  • EV miRNA profile, and EV protein profile were tracked.

Outcomes

EV miRNAs

CAT and HIIT produced distinct differentially expressed miRNA profiles.

CAT 67 DE miRNAs; HIIT 13 DE miRNAs versus rest.

Mixed

EV proteins

CAT and HIIT produced distinct differentially expressed protein profiles.

CAT 55 DE proteins; HIIT 70 DE proteins versus rest.

Mixed

EV concentration

HIIT showed a nonsignificant trend toward higher EV concentration.

Reported as not significant.

No clear change

Insights

  • This is useful as mechanistic background for acute HIIT biology, not as programming outcome evidence.
  • Heart-rate-based cycling HIIT can be matched in time to continuous aerobic training.

Limitations

  • Only five participants.
  • Male active sample only.
  • Acute molecular outcomes only.
  • Different workloads may confound modality comparisons.
  • EV tissue origins were inferred.

Safety

  • No adverse events were reported.
  • Participants completed physical examination, blood analysis, and adaptive training before the formal experiment.
  • Exercise was supervised by a professional coach with continuous real-time heart-rate monitoring; people unsuitable for physical training were excluded.